Composite

Part:BBa_K2922022:Design

Designed by: Xv Jiang   Group: iGEM19_XMU-China   (2019-10-13)


T7-RBS-cex-T7-RBS-bgl1A functioned in Kil secretion cassette with promoter J23109


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NotI site found at 818
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 451
    Illegal NgoMIV site found at 824
    Illegal NgoMIV site found at 1326
    Illegal NgoMIV site found at 3014
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 871
    Illegal SapI.rc site found at 954


Design Notes

Construct from existed parts according to Standard BioBrick Assembly.


Source

Cex is from Cellulomonas fimi and bgl1A comes from Saccharophagus degradans 2-40.

References